Download The Application of Laser Light Scattering to the Study of Biological Motion PDF

TitleThe Application of Laser Light Scattering to the Study of Biological Motion
Author
LanguageEnglish
File Size25.1 MB
Total Pages682
Document Text Contents
Page 1

The Application of Laser Light
Scattering to the Study of
Biological Motion

Page 2

NATO Advanced Science Institutes Series
A series of edited volumes comprising multifaceted studies of contemporary scientific
issues by some of the best scientific minds in the world, assembled in cooperation with
NATO Scientific Affairs Division.

This series is published by an international board of publishers in conjunction with.
NATO Scientific Affairs Division

A Life Sciences Plenum Publishing Corporation
B Physics New York and London

C Mathematical and D. Reidel Publishing Company
Physical SCiences Dordrecht, Boston, and London

0 Behavioral and Martinus Nijhoff Publishers
Social Sciences The Hague, Boston, and London

E Applied Sciences

F Computer and Springer Verlag
Systems Sciences Heidelberg, Berlin, and New York

G Ecological Sciences

Recent Volumes in Series A: Life Sciences

Volume 56-Advances in Vertebrate Neuroethology
edited by Jorg-Peter Ewert, Robert R. Capranica, and David J. Ingle

Volume 57-Biochemical and Biological Markers of Neoplastic Transformation
edited by Prakash P. Chandra

Volume 58-Arterial Pollution: An Integrated View on Atherosclerosis
edited by H. Peeters, G. A. Gresham, and R. Paoletti

Volume 59-The Applications of Laser Light Scattering to the Study of Biological Motion
edited by J. C. Earnshaw and M. W. Steer

Volume 60-The Use of Human Cells for the Evaluation of Risk from Physical
and Chemical Agents
edited by Amleto Castellani

Volume 61-Genetic Engineering in Eukaryotes
edited by Paul F. Lurquin and Andris Kleinhofs

Volume 62-Heart Perfusion, Energetics, and Ischemia
edited by Leopold Dintenfass, Desmond G. Julian, and
Geoffrey V. F. Seaman

Page 341

348 M. C. A. GRIFFIN AND M. ANDERSON

Q)
<.l
c:
co
.0

<5
'" .0
~

8.0

6.0

4.0

2.0

.-. Absorbance at 341nm
0--0 Absorbance at 280nm

0.0~;2----;4----!6----~8--~1~0~~1~2~~1~4--~1=6--~1=8--~2~0--~2~2----2L4----2L6----2L8--~30
Fraction number

Figure 1. Absorbance profiles of fractions eluted from the CPG
column after application of 1 ml skim milk.

casein micelles were eluted first, followed by soluble casein and
whey proteins.

The fractions were then examined by photon correlation
spectroscopy using a Malvern multibit correlator type K7025 and
spectrometer with a Spectra-Physics ReNe 15mW laser. Samples from
the fractions were diluted into SMUF, to a concentration at which
there were no observable effects of multiple scattering, and the
temperature of the cuvettes was kept at 20oc. Auto-correlation
functions were obtained at 900 scattering angle, and the normal-
ised correlation data, g(2) (T) ,were analysed by a least squares
fit of In(g(2) (T)-l) to a third order polynomial:

ln A - fT
o

+

omitting higher or~er terms, and weighting the data as (g(2) (T)-1)2.
From the value of f the diffusion coefficient was calculated from
D = f/K2 where K = 4nn . sin 8/2, the length of the scattering
vector. An I average' A diffusion coefficient, 0, of particles in
each fraction was obtained using a version of the method described
by Brown and Pusey (1974) in which the values of D were linearly
extrapolated to fT max = O.

Page 342

FRACTIONATION OF CASEIN MICELLES 349

I 1 6 Size from 'Nanosizer' l I E300'" Hydrodynamic diameter 2
jI-t'i ~ 1/

300f \\ J:'2 4 6 ~. "r-J! 6 1012141618202224262830 I L-____ --'FC'r ... action n.~u~m~be~r ___ ~ ,
E 1\ ~/2-,'2/d
s ~ /
I hy\ /,-'1
~ ~ ~~

.300

~

'II)

N

E

r-
o
x

.~ ~~~=-:r~-
[ 200 Q/Q' I 'i ...... r .200 ,0
"0 / I\
~ ~~N ~
~ )£-( '\

}

)Il-~/ I,,-¥,I"'-i
f"J/ A-A Hydronamic diameter \

'\ / :&.----
~~ ~ [}----o Diffusion coefficient D20,w ,~

100L-*2-~4~-*6-~8~-~10~~1~2-~14~~1~6-~18~~2~0~~2~2--f~~~2~6-~28~~30jOO
Fraction number

Figure 2. Profile of diffusion coefficients and hydrodynamic
diameters obtained for each fraction from the same
column-run described in Figure 1.

These average values of the diffusion coefficients of the
casein particles in each fraction are shown in Figure 2 which also
shows the hydrodynamic diameters (obtained assuming that the
Stoke's relationship holds: d kT).

3111115

For the purpose of comparison, Figure 2 shows results for the
hydrodynamic diameters obtained using an automatic photon
correlation spectrometer (a Coulter 'Nano-Sizer', kindly made
available to us by Express Dairy Foods Ltd) which operates on the
same prinCiples as the Malvern spectrometer used in the present
study, but which produces size and polydispersity data without the
need for software, according to unrevealed cabalistic formulae!
(The Coulter Nano-Sizer, 1980).

Page 681

704

Pollen tubes, 360, 383, 541
LLS study, 386

Polydisperse analysis
various shaped particles, 71

Polydisversity, 53, 58, 69, 94
183

Polyelectrolytes, 89, 115
Poly-L-lysine, 117
Polysomes, 46
Polyoxythylene surfactants,

341
P-protein, 149
Pressure flow, 502
Profile analysis, 53
Programs for multiexponentials,

67
Prolate ellipsoid, 73
Protein network, 495
Pseudopodia, 519
Puromycin, 46

Quarter wave plate, 158

Radius of gyration, 18, 70
Rayleigh-Debye approximation,

18
Rayleigh ratio, 336

of micellar solutions, 345
Red blood cells

ghosts, 112
Reference-beam method, 25
Refractive index, 153
Relaxing solution, 479
Reptilase, 186
Resolution of

multiexponentials, 66
Restricted rotation, 250
Reynolds number, 150, 562
Rheological properties of

cytoplasm, 530
Ribosomes, 34, 38
Ripplon, 275
RNA, 34, 46
Rod polymers, 71
Root hairs, 360
Rotational diffusion, 21

constant, 182
Rotation model, 545
Rotational motion, 134, 253

Run twiddle
motion, 609
two step model, 621

Saclay distribution, 663
Sampling theorem, 99, 462
Sampling time

discrete, 81
Samples

total, 78
Sarcomere length, 486
Sartorius muscle, 485
Scaling, 434, 632
Scanning mirror, 257
Scattered intensity, 335
Scattered electric field, 12
Scattered intensity, 12, 343

absolute calibration, 27
light level, 47

INDEX

Scattering angle, 151
Scattering cross section, 266
Scattering vector, 11
Scattering volume, 22, 46, 151
Scattering wave number, 154
Secondary scatter, 212
Second virial coefficient, 70

correction, 69
Secretion, 111
Secretory cells, 360
Self-diffusion coefficient, 28
Self-focusing, 494
Semiflexible worm-like chain,

74
Sherwood number, 567
Shuttle streaming, 501
Sialic acid, 113
Siegert relation, 179
Sieve elements, 149
Sieve plates, 149
Sieve pores, 149
Sieve tubes 150
Simplex method multiexponentials,

67
Single clipped correlator, 177
Sliding filament theory, 409
Small-scale hydrodynamics, 562
Smoluchowski equation, 91
Snell's law, 156
Sodium dodecylsulfate, 197

Page 682

INDEX

Software, 78
Sol * gel transformations, 501
Spectrin, 263
Spectrometer

small angle, 624
Spectrum analysers, 105, 125
Spermatozoa, 562, 631
Sperm chemotaxis, 602
Sperm motility, 659
Sphere, 73
Splines, 184, 434
Staminal hair cells, 159
Static yield strength, 394,

395, 398, 399, 401
Statistical distortion, 144
Stokes-Einstein relation, 180
Strain birefringence, 522
Streak camera, 251
Surface charge density, 91,

367, 369, 374
Surface elasticity, 288
Surface tension, 276
Surface viscoelasticity, 277
Synchrontron radiation, 251
Systems-intensive, 688

Tachypleus polyphenus, 478
Technique-intensive, 688
Temperature-controlled

scattering celL, 340
Thick filaments, 409
Thin disc, 73
Thin filaments, 409
Thin rod, 73
Thixotropic properties, 224
Thrombin, 185
Time correlation function, 54
Time dependent flows, 135
Time resolved fluorescence

emission, 247
Time to amplitude converter,

251
Tradescantia virginiana, 159
Transit time broadening, 93,

127
Translational diffusion

coefficient, 14, 180
Translational movement, 43
Translocation, 149
Transverse shear viscosity, 328

Trypsin, 113
Turgor, 150
Turntable, 85

Unibus, 85
Unimodal distributions, 58

Variable characteristic
linewidth, 64

Vascular bundles, 149
Velocity averaging, 129
Velocity profile, 522, 534
Vesicle, 108, 112
Vesicles, 36, 150, 201, 359

dynamics, 363
production rate, 384
transport, 511

Video-enhanced contrast
microscopy, 536

Video intensification
microscopy, 165

Videomicroscopy, 389, 390,
392

705

Viscosity, 44, 91, 389, 390,
394, 395, 396, 397, 398

Water surface, 290
Weight average molecular weight,

70

Xylem, 149

Yield strength, 389, 394

Zeta potential, 91

Similer Documents