Download physiological effects of nitrate, light, and intertidal position on the red seaweeds mazzaella PDF

Titlephysiological effects of nitrate, light, and intertidal position on the red seaweeds mazzaella
LanguageEnglish
File Size2.2 MB
Total Pages88
Table of Contents
                            SKMBT_C364e18010816530
Bitterwolf Thesis FINAL DRAFT
	Thesis Research: The Genus Mazzaella
	Objectives and Hypothesis
	Site Description
	Species & Ploidy Identification
	Field Experiment
	Monitoring
	Photosynthetic Pigment Extraction Method
	Tissue Preparation
	Pigment Extraction
	HPLC Method for Chlorophylls, Carotenoids, and Xanthophylls
	Spectrophotometric Method for Phycobilin Content
	Lab Experiments
	Cultivation Vessels
	Experimental Design
	Statistical Analysis
	Field Experiment
	Laboratory Experiments
	Hypothesis Testing
                        
Document Text Contents
Page 1

PHYSIOLOGICAL EFFECTS OF NITRATE, LIGHT, AND INTERTIDAL POSITION
ON THE RED SEAWEEDS MAZZAELLA FLACCIDA AND MAZZAELLA SPLENDENS
_______________

A Thesis

Presented to the

Faculty of

Moss Landing Marine Laboratories

California State University Monterey Bay

_______________

In Partial Fulfillment

of the Requirements for the Degree

Master of Science

in

Marine Science

_______________

by

Stephan A. Bitterwolf

Fall 2017

Page 44

35

Pigments – Throughout the experiment, M. flaccida had higher zeaxanthin and lutein

proportions than M. splendens (Figure 21; See Appendix Table A13-A14). Chlorophyll a

allomer, chlorophyll a, and beta carotene increased over the 14 days for both species (Figure 22).

Alpha carotene did not change over the period, but was significantly greater in M. splendens than

M. flaccida. Phycoerythrin and phycocyanin content decreased over the 14 days for both species

(Figure 23). Finally, the beta/alpha carotene ratio differed significantly between species and

sample day (Fig 24).



Figure 21. Mean zeaxanthin and lutein proportion (±SE) by species and day. * Indicates
significant difference as determined by t-test.

* *

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36


Figure 22. Mean pigment area percent (±SE) by species and day sampled. * indicates significant
difference as determined by t-test.


Figure 23. Mean phycobilin content (mg/gFW ±SE) by species, pigment, and sample day. *
indicates significant difference as determined by t-test.

*

* *

*

* *

Page 87

Zeaxanthin Day 1 1 0.51 0.80 0.3812
Phycoerythrin Nutrients*Light 1 1 0.21 0.79 0.3824

Lutein Nutrients*Species*Day 1 1 0.47 0.78 0.3862
Lutein Nutrients*Species 1 1 0.46 0.76 0.39

Chlorophyll a
allomer

Light*Day 1 1 0.27 0.73 0.4006

Chlorophyll a
allomer

Nutrients*Light 1 1 0.26 0.71 0.4073

Phycoerythrin Light*Species*Day 1 1 0.19 0.71 0.4076
Phycocyanin Species*Day 1 1 0.24 0.54 0.4682
Phycocyanin Light*Species 1 1 0.24 0.54 0.4687
Zeaxanthin Nutrients*Light 1 1 0.32 0.50 0.4871

Phycoerythrin Nutrients*Light*Day 1 1 0.12 0.45 0.5066
Phycocyanin Nutrients*Light 1 1 0.20 0.45 0.5085

Lutein Day 1 1 0.27 0.44 0.5116
Chlorophyll a Nutrients*Species*Day 1 1 0.20 0.43 0.5168

Alpha Carotene Nutrients*Species*Day 1 1 0.32 0.40 0.5309
Beta Carotene Nutrients*Light*Day 1 1 0.26 0.35 0.5589

Lutein Species*Day 1 1 0.20 0.34 0.5645
Alpha Carotene Nutrients*Species 1 1 0.25 0.31 0.5803

Phycocyanin Nutrients*Light*Day 1 1 0.13 0.30 0.5875
Lutein Nutrients*Light*Species 1 1 0.17 0.29 0.5964

Beta Carotene Nutrients*Day 1 1 0.21 0.29 0.5974

Alpha Carotene
Nutrients*Light*Species*Da

y
1 1 0.21 0.26 0.6113

Chlorophyll a
allomer

Nutrients*Light*Species 1 1 0.09 0.25 0.6199

Alpha Carotene Nutrients*Light 1 1 0.19 0.24 0.6312
Zeaxanthin Light*Species*Day 1 1 0.14 0.21 0.6491

Chlorophyll a Nutrients*Day 1 1 0.10 0.20 0.6558
Alpha Carotene Nutrients*Light*Day 1 1 0.15 0.19 0.6704
Beta Carotene Nutrients*Light*Species 1 1 0.13 0.18 0.6728
Beta Carotene Nutrients*Light 1 1 0.13 0.18 0.6729

Beta Carotene
Nutrients*Light*Species*Da

y
1 1 0.13 0.18 0.6763

Phycoerythrin Light*Day 1 1 0.05 0.17 0.6832
Chlorophyll a

allomer
Nutrients*Day 1 1 0.06 0.17 0.6842

Phycocyanin Nutrients*Species*Day 1 1 0.07 0.17 0.6866
Beta Carotene Nutrients*Species 1 1 0.09 0.13 0.7217

Lutein
Nutrients*Light*Species*Da

y
1 1 0.08 0.13 0.7249

Page 88

Zeaxanthin Nutrients*Species 1 1 0.07 0.11 0.7402
Zeaxanthin Light 1 1 0.06 0.10 0.7596

Alpha Carotene Nutrients*Light*Species 1 1 0.07 0.09 0.7677
Zeaxanthin Light*Day 1 1 0.05 0.08 0.7836
Zeaxanthin Species*Day 1 1 0.04 0.07 0.7937

Phycoerythrin Nutrients*Day 1 1 0.02 0.06 0.8078
Beta Carotene Light*Species 1 1 0.04 0.06 0.8123
Phycoerythrin Light*Species 1 1 0.01 0.04 0.8389

Lutein Nutrients*Day 1 1 0.02 0.04 0.8415
Zeaxanthin Light*Species 1 1 0.02 0.03 0.8601

Lutein Light 1 1 0.02 0.03 0.867
Beta Carotene Nutrients 1 1 0.02 0.02 0.8769
Chlorophyll a

allomer
Species*Day 1 1 0.01 0.02 0.8864

Zeaxanthin Nutrients*Day 1 1 0.01 0.02 0.8939
Chlorophyll a Light*Species*Day 1 1 0.01 0.01 0.9112
Chlorophyll a Species*Day 1 1 0.01 0.01 0.916
Phycocyanin Nutrients*Species 1 1 0.00 0.01 0.9198
Zeaxanthin Nutrients*Light*Day 1 1 0.01 0.01 0.9205

Chlorophyll a Nutrients 1 1 0.00 0.01 0.9231
Phycocyanin Nutrients*Day 1 1 0.00 0.01 0.9353
Chlorophyll a

allomer
Nutrients*Light*Species*Da

y
1 1 0.00 0.01 0.9362

Zeaxanthin Nutrients*Species*Day 1 1 0.00 0.00 0.9483
Chlorophyll a Light*Species 1 1 0.00 0.00 0.9639
Chlorophyll a Nutrients*Light*Day 1 1 0.00 0.00 0.9685
Chlorophyll a

allomer
Nutrients 1 1 0.00 0.00 0.9694

Alpha Carotene Light*Species*Day 1 1 0.00 0.00 0.9711
Phycoerythrin Nutrients*Species 1 1 0.00 0.00 0.9715
Phycoerythrin Species*Day 1 1 0.00 0.00 0.9718

Zeaxanthin Nutrients 1 1 0.00 0.00 0.9738
Chlorophyll a

allomer
Nutrients*Light*Day 1 1 0.00 0.00 0.9947

Chlorophyll a
allomer

Nutrients*Species*Day 1 1 0.00 0.00 0.9976

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