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Titlemolecular mechanisms of senescence response to transforming growth factor-beta in liver cancer
LanguageEnglish
File Size43.7 MB
Total Pages357
Table of Contents
                            ozturk m. et. al 2009.pdf
	Senescence and immortality in hepatocellular carcinoma
		Introduction
		Senescence pathways
		Cyclin-dependent inhibitors as common mediators of senescence arrest
		Senescence of hepatocytes and chronic liver disease
		Senescence pathway aberrations and telomerase reactivation in hepatocellular carcinoma
		Senescence as an anti-tumor mechanism in hepatocellular carcinoma
		Concluding remarks
		AcknowledgmentS
		References
yuzugullu h. et. al. molecular cancer 2010.pdf
	Abstract
		Background
		Results
		Conclusion
	Background
	Results
		Classification of hepatocellular carcinoma cell lines into "well-differentiated" and "poorly differentiated" subtypes
		Expression TCF/LEF family of transcription factors
		Expression of Frizzled receptors and LRP co-receptors
		Differential expression of canonical and noncanonical Wnt ligands
		Autocrine canonical Wnt signaling in well differentiated hepatocellular carcinoma cell lines
		Canonical Wnt signaling is repressed in poorly differentiated hepatocellular carcinoma cells
		WNT5A inhibits canonical Wnt signaling in HCC cells
	Discussion
	Conclusion
	Methods
		Cell lines
		Reverse transcription-polymerase chain reaction (RT- PCR) analysis
		Wound-healing assay
		Immunocytochemistry
		Confocal microscopy
		Plasmids
		Transfections
		Generation of mutant b-catenin expressing SNU449-cl8 cell line
		Western blotting
		Statistical analysis
	List of abbreviations
	Competing interests
	Authors' contributions
	Authors' information
	Acknowledgements
	References
buentempo f. et. al. inv new drugs 2010.pdf
	Inhibition of Akt signaling in hepatoma cells induces apoptotic cell death independent of Akt activation status
		Abstract
		Introduction
		Materials and methods
			Materials
			Cell culture
			Western blotting analysis
			Akt kinase activity assay
			NCI-60 Sulforhodamine B (SRB) cytotoxicity assay
			Real-time cell growth surveillance by cell electronic sensing
			Fluorescence microscopy
			RT-PCR
		Results
			Akt hyperphosphorylation in Mahlavu HCC cells
			Determination of IC50 values for the HCC cells with SRB cytotoxicity assay
			Real-time, dynamic monitoring of cell growth in HCC cells treated with the inhibitors
			Cytotoxic effect of drugs on HCC cells through apoptosis
			Comparative analysis of PTEN in HCC cells
		Discussion
		References
                        

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