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TitleIntestinal Transport: Fundamental and Comparative Aspects
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Page 1

Proceedings in Life Sciences

Page 2

European Society for Comparative Physiology and Biochemistry
4th Conference, Bielefeld, September 8-11, 1982

Conference Organization

General Organizers
R. Gilles and H. Langer
Liege, Belgium/Bochum, FRG

Local Organizers
K. Immelman, E. Prove, and S. Sossinka
Bielefeld, FRG

Symposium Organizers
Intestinal Transport
M. Gilles-Baillien
Liege, Belgium

Hormones and Behaviour
J. Bal thazart
Liege, Belgium

Under the Patronage of

The Deutsche Forschungsgemeinschaft

The Department for Scientific Research of the
Bundesland Nordrhein Westfalen

The Paul-Martini-Stiftung der
Medizinisch Pharmazeutischen Studiengesellschaft
The University of Bielefeld
The University of Liege
The European Society for Comparative Physiology and Biochemistry

Page 191

Mechanism of Active Calcium Transport in Basolateral Plasma Membranes 183

Moore L, Fitzpatrick DF, Chen TS, Landon EJ (1974) Calcium pump activity of the renal plas-
mamembrane and renal micro somes. Biochim Biophys Acta 345 :405-418

Nellans HN, Kimbert DV (1978) Cellular and paracellular calcium transport in rat ileum. Effects
of dietary calcium. Am J PhysioI235:E716-E727

Nellans HN, Popovitch TE (1981) Calmodulin regulated, ATP driven calcium transport by baso-
lateral membranes of rat small intestine. J Bioi Chern 256:9932-9936

Nordin BEC (1976) Calcium, phosphate and magnesium metabolism. Churchill Livingstone,
Edinburgh London New York

Os CH van, Jonge HR de, Jong MD de, Gbijsen WEJM, Walters JALI (1980) Separation ofbaso-
lateral plasma membranes from smooth endoplasmic reticulum of the rat enterocyte by zonal
electrophoresis on density gradients. Biochim Biophys Acta 600:730-738

Rasmussen H, Fontaine 0, Matsumoto T (1981) Liponomic regulation of calcium transport by
1,25 (OH)2 D3. Ann NY Acad Sci 372:518-528

Sarkadi B (1980) Active calcium transport in human red cells. Biochim Biophys Acta 604:159-
190

Slater EC (1979) Measurement and importance of phosphorylation potentials: Calculation of free
energy of hydrolysis in cells. In: Colowick SP, Kaplan NO (eds) Methods in enzymology,
vol 55, part F, pp 235-245

Thomasset M, Molla A, Parkes 0, Demaille JG (1981) Intestinal calmodulin and calcium-binding
protein differ in their distribution and in the effect of vitamin D steroids on their concentra-
tion. FEBS Lett 127:13-16

Walling MW (1977) Intestinal Ca and phosphate transport: differential responses to vitamin D3
metabolites. Am J PhysioI233:E488-E494

Wasserman RH (1981) Intestinal absorption of calcium and phosphorus. Fed Proc 40:68-72
Wasserman RH, Tqylor AN (1963) Nonessentiality of sodium ions for intestinal calcium trans;

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Weber A (1966) Energized calcium transport and releasing factors. In: Sanadi DR (ed) Current

topics in bioenergetics. Academic Press, New York, pp 203-254

Page 192

The Small Intestinal Na +, D-Glucose Cotransporter:
a Likely Model

G.SEMENZA 1

The co-transport mechanism of the coupled movement of D-glucose and N a + across the
brush border membrane is now universally accepted (for a review, see Crane 1977).

The transport agency shows a stable asymmetry with respect to the plane of the
membrane, as demonstrated by its asymmetric inactivation (or reactivation) by little-
permeant or impermeant reagents. Comparison was made between little permeant
vs. very permeant reagents and between sealed, right-side out membrane vesicles vs.
deoxycholate-extracted membrane fragments (Klip et al. 1979a,b, 1980a,b). The Na +,
D-glucose cotransporter shows a functional asymmetry also (in addition, of course,
to that which can be imposed onto it by asymmetric distributions of substrate(s)
and/or by a Ll!Jt =F 0). In fact, in right-side out vesicles: (1) trans-inhibitions and
trans-stimulations by substrates are much larger in influx than in efflux experiments;
(2) D-glucose influx responds to Ll!Jt much more than its efflux; (3) the apparent ~
for efflux is more than one order of magnitude larger than the apparent ~ for
influx; (4) influx and efflux rates may differ by one order of magnitude when mea-
sured at equivalent, but mirrored, conditions (Kessler and Semenza 1983).

The observed asymmetric insertion of the cotransporter in the membrane obviously
agrees with current ideas on the biosynthesis and vectorial insertion ofintrinsic mem-
brane proteins. It rules out, also, freely "diffusing" or "tumbling over" carrier models
for its mode of operation, whereas it makes more likely a "gated channel" (Semenza
1982; see Crane and Dorando 1979, 1980, 1983), i.e., a "channel with multiple con-
formational states", which is a limiting case of the general channel mechanisms
(Uiuger 1980).

Small intestinal Na+, D-glucose cotransporter shows the functional characteristics
of a "mobile carrier": in particular, it shows counterflow. In addition, optimal bind-
ing of phlorizin to the cotransporter in vesicles requires, in addition to Na+, a Ll!Jt ~ 0
(negative inside the vesicles) which indicates that under the action of the electric
field a portion of the channel must move to allow phlorizin (which is uncharged,
Toggenburger et al. 1978) to bind.

It is possible to obtain some information on the nature of this "gate". As men-
tioned above, intravesicular D-glucose (and also Na+, but not both at the same time)
exert a strong transinhibition on the Na+-dependent out -+ in (influx) of D-glucose.

1 Laboratoriurn fiir Biochemie der Eidgenossischen Technischen Hochschule ZUrich, Universi-
tatsstrasse 16, CH-8092 Ziirich, Switzerland

Intestinal Transport
(ed. by M. Gilles-Baillien and R. Gilles)
© Springer-Verlag Berlin Heidelberg 1983

Page 382

Springer-Verlag
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Osmoregulation in Birds
1981. 42 figures. X, 203 pages
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Uptake Through the Gut - Evapomtion. - Function of the Kid-
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action Among the Excretory Organs. - A Brief Swvey of Hormones
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and of Egg-Laying. - References. - Systematic and Species Index. -
Subject Index.

Structural and Functional Aspects
of Enzyme Catalysis
32. Colloquium der Gesellschaft fUr Biologische Chemie
23. -25. April 1981 in MosbachlBaden

Editors: H. Eggerer, R. Huber
1981. 116 figures. Ix, 216 pages
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Contents: Mechanism of Enzyme Action. - Dynamics of Molecular
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Selected Topics of Enzyme Catalysis. - Subject Index.

Biochemistry of Differentiation
and Morphogenesis
33. Colloquium der Gesellschaft fUr Biologische Chemie
25.-27. Miirz 1982 in MosbachlBaden
Editor: L. Jaenicke
1982. 158 figures. XI, 301 pages.
ISBN 3-540-12010-6

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Page 383

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