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Idohna Leah J. Buendia January 19, 2011
Bio 120 A-2L Sir Michael Delomen
Cell Respiration
Liver is the largest internal organ of the body. It has many functions including
metabolism. Metabolism and some other function require energy. This energy is provided by the
liver mitochondria because the mitochondria are the power house of energy. Liver cells have
maximum number of mitochondria (1000-2000 per cell). The enzymes in the liver cell
mitochondria help in detoxifying ammonia which is produce as waste product of metabolism.
This is the reason that liver cells have high number of mitochondria.
Among the six test tubes, only set-up 4 was heated and got the slowest respiration rate
mainly because enzymes in set-up 4 were denatured due to heat given. This proves that enzymes
function as catalysts of reactions. Same goes with set-up 3 where the one being tested if there is
an involvement of the succinate in the reaction. And it was clearly seen that DPIP was not
reduced since there was no presence of succinate. Instead DPIP was oxidized. Thus there was an
increase in the absorbance value.
Liver homogenate was used as the source of the succinate dehydrogenase enzyme.
Cyanide on the other hand acts as an inhibitor and is irreversible. Cyanide would prevent the
production of ATP from respiration because it would bind with complex IV of the electron
transport chain/system (ETS). Thus if there is no production of ATP, respiration will not
continue. Malonate would also act as inhibitor but this it is reversible specifically competitive
inhibitor. This is so because malonate has structural analog of the succinate. Malonate would
inhibit and bind with complex II of also ETS. Thus there would not be transfer of electron and so
http://www.blurtit.com/q999311.html
http://www.blurtit.com/q138434.html
Idohna Leah J. Buendia January 19, 2011
Bio 120 A-2L Sir Michael Delomen
Cell Respiration
Liver is the largest internal organ of the body. It has many functions including
metabolism. Metabolism and some other function require energy. This energy is provided by the
liver mitochondria because the mitochondria are the power house of energy. Liver cells have
maximum number of mitochondria (1000-2000 per cell). The enzymes in the liver cell
mitochondria help in detoxifying ammonia which is produce as waste product of metabolism.
This is the reason that liver cells have high number of mitochondria.
Among the six test tubes, only set-up 4 was heated and got the slowest respiration rate
mainly because enzymes in set-up 4 were denatured due to heat given. This proves that enzymes
function as catalysts of reactions. Same goes with set-up 3 where the one being tested if there is
an involvement of the succinate in the reaction. And it was clearly seen that DPIP was not
reduced since there was no presence of succinate. Instead DPIP was oxidized. Thus there was an
increase in the absorbance value.
Liver homogenate was used as the source of the succinate dehydrogenase enzyme.
Cyanide on the other hand acts as an inhibitor and is irreversible. Cyanide would prevent the
production of ATP from respiration because it would bind with complex IV of the electron
transport chain/system (ETS). Thus if there is no production of ATP, respiration will not
continue. Malonate would also act as inhibitor but this it is reversible specifically competitive
inhibitor. This is so because malonate has structural analog of the succinate. Malonate would
inhibit and bind with complex II of also ETS. Thus there would not be transfer of electron and so
http://www.blurtit.com/q999311.html
http://www.blurtit.com/q138434.html
