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TitleCrafting with Livings
LanguageEnglish
File Size5.7 MB
Total Pages104
Table of Contents
                            Remerciements
Abstract|Résumé
List of Figures
Introduction
1 Crafting with Livings
	1.1 Biolaboratories
	1.2 Correspondence
		Transparent Wood
		Physarum polycephalum overruns wooden houses
		Unidentified fungal bodies
2 Gesturing in the Field
	2.1 Research trajectory
	2.2 Methodological questions
	2.3 Polite inquiry
3 Bridging Crafting with Living
	3.1 Crafting
		Craft of tissue culture, tacit knowledge and ‘new’ media
		Broken pieces, sloppiness, hacking and problem solving
	3.2 Living
		Bees, ceramics and the liveliness of materials
	3.3 Bridging
4 Calibration
	4.1 Calibration
	4.2  Gestures and hands
	4.3 Cellular anthropology
Conclusion
	Limits
Bibliography
                        
Document Text Contents
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Contents

Remerciements ............................................................................................................................................. iv

Abstract|Résumé ........................................................................................................................................... v

List of Figures .............................................................................................................................................. vi

Introduction ................................................................................................................................................... 1

1 Crafting with Livings ......................................................................................................................... 11

1.1 BIOLABORATORIES ........................................................................................................................ 11

1.2 CORRESPONDENCE ......................................................................................................................... 20

Transparent Wood .............................................................................................................................. 22

Physarum polycephalum overruns wooden houses ............................................................................ 24

Unidentified fungal bodies ................................................................................................................. 26

2 Gesturing in the Field ......................................................................................................................... 31

2.1 RESEARCH TRAJECTORY ................................................................................................................ 31

2.2 METHODOLOGICAL QUESTIONS ..................................................................................................... 34

2.3 POLITE INQUIRY ............................................................................................................................. 40

3 Bridging Crafting with Living ........................................................................................................... 45

3.1 CRAFTING ....................................................................................................................................... 45

Craft of tissue culture, tacit knowledge and ‘new’ media .................................................................. 45

Broken pieces, sloppiness, hacking and problem solving .................................................................. 54

3.2 LIVING ............................................................................................................................................ 59

Bees, ceramics and the liveliness of materials ................................................................................... 59

3.3 BRIDGING ....................................................................................................................................... 65

4 Calibration .......................................................................................................................................... 68

4.1 CALIBRATION ................................................................................................................................. 68

4.2 GESTURES AND HANDS .................................................................................................................. 72

4.3 CELLULAR ANTHROPOLOGY .......................................................................................................... 77

Conclusion .................................................................................................................................................. 86

LIMITS ..................................................................................................................................................... 88

Bibliography ............................................................................................................................................... 90

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First, I proceeded to remove the media by

aspirating the liquid with a glass needle connected to

a vacuum tube. After, I had to wash my cells with

PBS. This meant to add PBS with a pipette gun and

swirl the PBS gently in circles in the Petri dish to

remove any drop of media left – this step was crucial

because media renders Trypsin inactive. This step

had to be done gently to avoid damaging the cells

during the washing. After the washing step, I added

the Trypsin to the Petri dish and incubated the cells

for 5 minutes. Trypsin is a scissor-like enzyme which

serves to cut away the connective tissue grown by

cells which helps them adhere to the plastic dish.

During that time, I had a timer on my phone to

calculate the 5 minutes, I prepared a 15 ml Falcon

tube with 5 ml of media and put away the bottle PBS

and Trypsin, respectively on a shelf and in the fridge. Hearing the beep of the timer reaching its

end, I could remove my cells from the incubator and bring them back into the safe space of the

biosafety hood. Petri dishes were never to be opened outside of the hood to avoid the

aforementioned contamination. With the pipette gun and a 10 ml pipette tip, I aspirated the mixture

of cells and trypsin and splashed it back on the Petri dish, which I held at a 45° angle. This helped

to detach the last cells still partially bonded to the plastic. After this gesture, I proceeded to empty

the pipette full of cell-trypsin mixture inside the pre-prepared falcon tube containing 5 ml of media.

This mixture allowed to neutralize the cutting effect of the trypsin which could damage the cells

over a longer period of time. By getting up and walking to the counter, I could place this 15 ml

falcon tube in the centrifuge which ran for 3 minutes at 1000 RPM. Little was left to do once I

reached this stage, though these last steps were crucial. Coming out of the centrifuge, the cells had

formed a pellet at the bottom of the tube. Bringing the tube back into the biosafety hood, using

ethanol to ensure sterility as with every removal and entry into the hood, I had to remove the

Tripsin-media mixture without touching the pellet of cells at the bottom of the vial. I used the same

glass needles first used to aspirate the cell media. The long thin head of the needle had to move

Figure 18: Cell pellets at the bottom of 15ml falcon

tubes after spinning in the centrifuge

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http://www.theguardian.com/science/2015/nov/18/%20%20%09biohackers-strange-world-diy-biology
http://www.theguardian.com/science/2015/nov/18/%20%20%09biohackers-strange-world-diy-biology

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